An enzymatic virus-like particle assay for sensitive detection of virus entry
نویسندگان
چکیده
منابع مشابه
Standardization of an Enzyme-Linked Immunosorbent Assay for Detection of Infectious Bronchitis Virus Antibody.
An indirect enzyme–linked immunosorbent assay (ELISA) was developed for screening of antibody to avian infectious bronchitis virus (IBV). Antigen was prepared from whole-purified IBV Massachusetts serotype (BR 801 strain). Optimum dilution with minimum background for antigen concentration, rabbit anti-chicken conjugate and sera in developed ELISA was determined 0.1μg/ml, 1:3000 and 1:100, respe...
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Morphological investagations were carried out on basophilic intranuclear and cytoplasmic inclusion bodies in epithelial cells of the hair bulbs of anagen hair follicles in wild foxes affected with an abnormal hair coat condition. The inclusions , which were DNA-positive, contained numerous minute virus-like particles ("FMVP"). The particles, having a diameter of approximately 13 nm, had an arra...
متن کاملstandardization of an enzyme-linked immunosorbent assay for detection of infectious bronchitis virus antibody.
an indirect enzyme–linked immunosorbent assay (elisa) was developed for screening of antibody to avian infectious bronchitis virus (ibv). antigen was prepared from whole-purified ibv massachusetts serotype (br 801 strain). optimum dilution with minimum background for antigen concentration, rabbit anti-chicken conjugate and sera in developed elisa was determined 0.1μg/ml, 1:3000 and 1:100, respe...
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ژورنال
عنوان ژورنال: Journal of Virological Methods
سال: 2010
ISSN: 0166-0934
DOI: 10.1016/j.jviromet.2009.10.020